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mouse antihuman mpo monoclonal antibody  (Bio-Rad)


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    Structured Review

    Bio-Rad mouse antihuman mpo monoclonal antibody
    Mouse Antihuman Mpo Monoclonal Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+antihuman+mpo+monoclonal+antibody/Mouse+anti+Human+Myeloperoxidase/pm40076841-164-25-30
    Average 95 stars, based on 144 article reviews
    mouse antihuman mpo monoclonal antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Control:

    Article Title: Enhanced Formation and Disordered Regulation of NETs in Myeloperoxidase-ANCA–Associated Microscopic Polyangiitis
    Article Snippet: .. For control, mouse antihuman MPO monoclonal antibody (AbD Serotec, Düsseldorf, Germany) was used instead of the IgG samples. ..

    Article Title: Enhanced Formation and Disordered Regulation of NETs in Myeloperoxidase-ANCA–Associated Microscopic Polyangiitis
    Article Snippet: Recombinant human MPO (1 μ g/ml or 100 μ g/ml) (Creative BioMart, Shirley, NY) was added to the IgG samples obtained from patients with MPO-ANCA–associated MPA, and then the NET induction assay was performed as described earlier. .. For control, mouse antihuman MPO monoclonal antibody (AbD Serotec, Düsseldorf, Germany) was used instead of the IgG samples. ..

    other:

    Article Title: Novel Methods for the Analysis of Serum NET Remnants: Evaluation in Patients with Severe COVID-19.
    Article Snippet: To quantify MPO−DNA complexes, 96-well polystyrene Nunc Maxisorp plates (Sigma Aldrich, St. Louis, MO, USA) were coated overnight at 4 ◦C with 5 μg/mL of mouse antihuman MPO monoclonal antibody (Biorad, Hercules, CA, USA) diluted in 0.1 M carbonate buffer, pH 9.6.



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    Neutrophil extracellular traps (NETs) contain selected enzymes from neutrophil cytoplasmic granules . Top panel shows human IgG staining (green), and bottom panel shows overlay of human IgG (green) and DNA staining (white). (A–D) Stainings of NETs from phorbol 12-myristate 13-acetate-activated neutrophils. DNA staining (white) shows NETs as elongated fibers. Bar represents 50 µm. (A) Staining with anti-myeloperoxidase <t>(MPO)-IgG</t> (green) shows NETs. (B) Anti-proteinase <t>3</t> <t>(PR3)-antibodies</t> do not stain NETs (green), but label cell bodies of netting neutrophils. (C) Anti-lactoferrin (LF) antibodies and (D) anti-neutrophil elastase (NE)-IgG stain NETs. (E–H) Staining of naïve neutrophils along with spontaneous NETs. DNA staining shows nuclei of neutrophils and spontaneously formed NETs. Bar represents 20 µm. (E) Anti-MPO-IgG stained spontaneously formed NET along with the cytoplasm of naïve cells. (F) Anti-PR3-IgG stained the cytoplasm of naïve cells but not spontaneously formed NET. (G) Anti-LF antibodies and (H) anti-NE antibodies stained the cytoplasm of naïve cells and spontaneously formed NET. Images are representative of at least three independent experiments.
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    Neutrophil extracellular traps (NETs) contain selected enzymes from neutrophil cytoplasmic granules . Top panel shows human IgG staining (green), and bottom panel shows overlay of human IgG (green) and DNA staining (white). (A–D) Stainings of NETs from phorbol 12-myristate 13-acetate-activated neutrophils. DNA staining (white) shows NETs as elongated fibers. Bar represents 50 µm. (A) Staining with anti-myeloperoxidase <t>(MPO)-IgG</t> (green) shows NETs. (B) Anti-proteinase <t>3</t> <t>(PR3)-antibodies</t> do not stain NETs (green), but label cell bodies of netting neutrophils. (C) Anti-lactoferrin (LF) antibodies and (D) anti-neutrophil elastase (NE)-IgG stain NETs. (E–H) Staining of naïve neutrophils along with spontaneous NETs. DNA staining shows nuclei of neutrophils and spontaneously formed NETs. Bar represents 20 µm. (E) Anti-MPO-IgG stained spontaneously formed NET along with the cytoplasm of naïve cells. (F) Anti-PR3-IgG stained the cytoplasm of naïve cells but not spontaneously formed NET. (G) Anti-LF antibodies and (H) anti-NE antibodies stained the cytoplasm of naïve cells and spontaneously formed NET. Images are representative of at least three independent experiments.
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    Bio-Rad mouse monoclonal antihuman mpo
    Neutrophil extracellular traps (NETs) contain selected enzymes from neutrophil cytoplasmic granules . Top panel shows human IgG staining (green), and bottom panel shows overlay of human IgG (green) and DNA staining (white). (A–D) Stainings of NETs from phorbol 12-myristate 13-acetate-activated neutrophils. DNA staining (white) shows NETs as elongated fibers. Bar represents 50 µm. (A) Staining with anti-myeloperoxidase <t>(MPO)-IgG</t> (green) shows NETs. (B) Anti-proteinase <t>3</t> <t>(PR3)-antibodies</t> do not stain NETs (green), but label cell bodies of netting neutrophils. (C) Anti-lactoferrin (LF) antibodies and (D) anti-neutrophil elastase (NE)-IgG stain NETs. (E–H) Staining of naïve neutrophils along with spontaneous NETs. DNA staining shows nuclei of neutrophils and spontaneously formed NETs. Bar represents 20 µm. (E) Anti-MPO-IgG stained spontaneously formed NET along with the cytoplasm of naïve cells. (F) Anti-PR3-IgG stained the cytoplasm of naïve cells but not spontaneously formed NET. (G) Anti-LF antibodies and (H) anti-NE antibodies stained the cytoplasm of naïve cells and spontaneously formed NET. Images are representative of at least three independent experiments.
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    mouse monoclonal antihuman mpo - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Neutrophil extracellular traps (NETs) contain selected enzymes from neutrophil cytoplasmic granules . Top panel shows human IgG staining (green), and bottom panel shows overlay of human IgG (green) and DNA staining (white). (A–D) Stainings of NETs from phorbol 12-myristate 13-acetate-activated neutrophils. DNA staining (white) shows NETs as elongated fibers. Bar represents 50 µm. (A) Staining with anti-myeloperoxidase (MPO)-IgG (green) shows NETs. (B) Anti-proteinase 3 (PR3)-antibodies do not stain NETs (green), but label cell bodies of netting neutrophils. (C) Anti-lactoferrin (LF) antibodies and (D) anti-neutrophil elastase (NE)-IgG stain NETs. (E–H) Staining of naïve neutrophils along with spontaneous NETs. DNA staining shows nuclei of neutrophils and spontaneously formed NETs. Bar represents 20 µm. (E) Anti-MPO-IgG stained spontaneously formed NET along with the cytoplasm of naïve cells. (F) Anti-PR3-IgG stained the cytoplasm of naïve cells but not spontaneously formed NET. (G) Anti-LF antibodies and (H) anti-NE antibodies stained the cytoplasm of naïve cells and spontaneously formed NET. Images are representative of at least three independent experiments.

    Journal: Frontiers in Immunology

    Article Title: Neutrophil Extracellular Traps Contain Selected Antigens of Anti-Neutrophil Cytoplasmic Antibodies

    doi: 10.3389/fimmu.2017.00439

    Figure Lengend Snippet: Neutrophil extracellular traps (NETs) contain selected enzymes from neutrophil cytoplasmic granules . Top panel shows human IgG staining (green), and bottom panel shows overlay of human IgG (green) and DNA staining (white). (A–D) Stainings of NETs from phorbol 12-myristate 13-acetate-activated neutrophils. DNA staining (white) shows NETs as elongated fibers. Bar represents 50 µm. (A) Staining with anti-myeloperoxidase (MPO)-IgG (green) shows NETs. (B) Anti-proteinase 3 (PR3)-antibodies do not stain NETs (green), but label cell bodies of netting neutrophils. (C) Anti-lactoferrin (LF) antibodies and (D) anti-neutrophil elastase (NE)-IgG stain NETs. (E–H) Staining of naïve neutrophils along with spontaneous NETs. DNA staining shows nuclei of neutrophils and spontaneously formed NETs. Bar represents 20 µm. (E) Anti-MPO-IgG stained spontaneously formed NET along with the cytoplasm of naïve cells. (F) Anti-PR3-IgG stained the cytoplasm of naïve cells but not spontaneously formed NET. (G) Anti-LF antibodies and (H) anti-NE antibodies stained the cytoplasm of naïve cells and spontaneously formed NET. Images are representative of at least three independent experiments.

    Article Snippet: Primary and secondary antibodies were used at the following concentrations: 5 µg/ml mouse antihuman MPO antibody (Hycult Biotech, Clone 266-6K1, product number: HM2164), 5 µg/ml of mouse antihuman PR3 antibody (Clone WGM2) , 2.5 µg/ml rabbit antihuman MPO antibody (DAKO, product number: A0398), 5 µg/ml rabbit antihuman lactoferrin (LF) antibody (Sigma-Aldrich, product number: L3262), 5 µg/ml mouse antihuman neutrophil elastase (NE) (DAKO, Clone NP57, product number: M0752), 5 µg/ml of Alexa Fluor-546 goat anti-rabbit IgG, 10 µg/ml of Alexa Fluor-488 goat anti-mouse IgG, and 10 µg/ml Alexa Fluor-555 goat-antihuman IgG (all Life Technologies).

    Techniques: Staining